Journal: Molecular microbiology

Article Title: Bactericidal peptidoglycan recognition protein induces oxidative stress in Escherichia coli through a block in respiratory chain and increase in central carbon catabolism

doi: 10.1111/mmi.13733

Figure Lengend Snippet: (A) Mutants with increased resistance to PGRP killing were identified by three-stage screening of the entire Keio collection of single gene deletion mutants (Fig. S1 and Table S1) and here the survival of the parental strain (BW25113) and mutants following 3-hr incubation with 200 µg/ml of BSA (as a control) or PGRP is shown. Gene products, their functions, and numerical data are shown in Table S1. (B) Mutants for the key genes for the respiratory chain and TCA cycle, and their regulators (cyaA and crp) were constructed in MG1655 and their sensitivity to killing by 100 µg/ml of PGRP was similarly tested. The results are means of 3 experiments, expressed as percent of initial inoculum (100%) + SEM; ^ P≤0.05, ^^ P<0.001, numbers of viable bacteria (colony forming units) of Δ mutants versus parental strain (t-test).

Article Snippet: The entire Keio collection of 3884 E. coli K-12 in-frame single-gene knockout mutants was obtained from the National BioResource Project, National Institute of Genetics, Japan ( Baba et al ., 2006 ).

Techniques: Incubation, Control, Construct, Bacteria

Journal: Molecular microbiology

Article Title: Bactericidal peptidoglycan recognition protein induces oxidative stress in Escherichia coli through a block in respiratory chain and increase in central carbon catabolism

doi: 10.1111/mmi.13733

Figure Lengend Snippet: (A) Time kinetics of changes of H2O2 in E. coli MG1655 treated with 100 µg/ml BSA or PGRP, or with 100 µM paraquat. (B) H2O2 in E. coli BW25113 and in the indicated deletion mutants from Keio collection treated with 100 µg/ml BSA or PGRP for 15 min. (C) H2O2 in E. coli MG1655 and in the indicated deletion mutants constructed in our laboratory treated with 100 µg/ml BSA or PGRP for 15 min. The results are means of 3–4 experiments ± SEM (SEM were within symbols if not visible); * P<0.05, ** P<0.001, PGRP vs BSA; +P<0.05, ++P<0.001, paraquat vs BSA; ^ P<0.05, ^^ P<0.001, PGRP-treated mutant vs parental strain.

Article Snippet: The entire Keio collection of 3884 E. coli K-12 in-frame single-gene knockout mutants was obtained from the National BioResource Project, National Institute of Genetics, Japan ( Baba et al ., 2006 ).

Techniques: Construct, Mutagenesis

Journal: Molecular microbiology

Article Title: Bactericidal peptidoglycan recognition protein induces oxidative stress in Escherichia coli through a block in respiratory chain and increase in central carbon catabolism

doi: 10.1111/mmi.13733

Figure Lengend Snippet: Parental E. coli or the indicated deletion mutants from Keio collection (BW25113) or from our laboratory (MG1655) were treated with 100 µg/ml BSA or PGRP for 15 min (A) or 5 min (B and C). The results are means of 3–4 experiments + SEM; * P<0.05, ** P<0.001, PGRP vs BSA; ^ P<0.05, ^^ P<0.001, mutant vs parental strain.

Article Snippet: The entire Keio collection of 3884 E. coli K-12 in-frame single-gene knockout mutants was obtained from the National BioResource Project, National Institute of Genetics, Japan ( Baba et al ., 2006 ).

Techniques: Mutagenesis

Journal: Molecular microbiology

Article Title: Bactericidal peptidoglycan recognition protein induces oxidative stress in Escherichia coli through a block in respiratory chain and increase in central carbon catabolism

doi: 10.1111/mmi.13733

Figure Lengend Snippet: E. coli and the indicated deletion mutants from Keio collection (BW25113) or from our laboratory (MG1655) were treated with 100 µg/ml BSA or PGRP for 30 min. The results are means of 3 experiments + SEM; * P<0.05, ** P<0.001, PGRP vs BSA; ^ P<0.05, mutant vs parental strain.

Article Snippet: The entire Keio collection of 3884 E. coli K-12 in-frame single-gene knockout mutants was obtained from the National BioResource Project, National Institute of Genetics, Japan ( Baba et al ., 2006 ).

Techniques: Mutagenesis

Journal: Nature genetics

Article Title: Genomic and functional analyses of Mycobacterium tuberculosis strains implicate ald in D-cycloserine resistance

doi: 10.1038/ng.3548

Figure Lengend Snippet: Unexplained drug resistance in 498 strains of M. tuberculosis from South Africa and China. The total number of strains assayed for phenotypic resistance to each drug is shown, along with the number of phenotypically resistant (R) and phenotypically susceptible (S) strains. The number of strains with phenotypic drug resistance but without a match to known resistance-associated mutations is also given.

Article Snippet: An ald knockout mutant (Δ ald ) of M. tuberculosis (JHU2780-209) was obtained from the Johns Hopkins TARGET mutant collection .

Techniques:

Journal: Nature genetics

Article Title: Genomic and functional analyses of Mycobacterium tuberculosis strains implicate ald in D-cycloserine resistance

doi: 10.1038/ng.3548

Figure Lengend Snippet: Convergent evolution of loss-of-function mutations in ald in MDR and XDR M. tuberculosis . A) Positions of insertions, deletions, and nonsense mutations in ald . Insertions are marked as blue triangles while deletions and nonsense mutations are marked as red triangles. Size of the indel or details of nonsense mutation are shown above or below each triangle. The location of the frameshift in BCG is also included. B) Phylogeny of lineage 4 strains. Inner tick marks denote MDR (gray) and XDR (black) M. tuberculosis . Outer tick marks denote insertions (blue) and deletions or nonsense mutations (red).

Article Snippet: An ald knockout mutant (Δ ald ) of M. tuberculosis (JHU2780-209) was obtained from the Johns Hopkins TARGET mutant collection .

Techniques: Mutagenesis

Journal: Nature genetics

Article Title: Genomic and functional analyses of Mycobacterium tuberculosis strains implicate ald in D-cycloserine resistance

doi: 10.1038/ng.3548

Figure Lengend Snippet: Alanine metabolism pathway in M. tuberculosis . L-alanine-dehydrogenase catalyzes the NAD-dependent interconversion between L-alanine and pyruvate. Under aerobic conditions this reaction allows for the utilization of L-alanine as a nitrogen source, whereas under hypoxic conditions this reaction allows for NADH recycling to NAD + . Separately, the initial step of peptidoglycan synthesis involves conversion of L-alanine to D-alanine by alanine racemase. Two D-alanine molecules are joined by D-ala-D-ala ligase to produce the dipeptide D-alanine-D-alanine, which is subsequently incorporated into the pentapeptide chain of the peptidoglycan cell wall. Both alanine racemase and D-ala-D-ala ligase are competitively inhibited by D-cycloserine, an analog of D-alanine.

Article Snippet: An ald knockout mutant (Δ ald ) of M. tuberculosis (JHU2780-209) was obtained from the Johns Hopkins TARGET mutant collection .

Techniques:

Journal: Nature genetics

Article Title: Genomic and functional analyses of Mycobacterium tuberculosis strains implicate ald in D-cycloserine resistance

doi: 10.1038/ng.3548

Figure Lengend Snippet: Loss-of-function of L-alanine dehydrogenase ( ald ) confers increased resistance to D-cycloserine in vitro. The minimum inhibitory concentration (MIC) to D-cycloserine was determined for each strain on Löwenstein–Jensen media. Testing was done in three independent experiments (assays #1–#3) to establish reproducibility. Dashes indicate those strains were not tested during that experiment.

Article Snippet: An ald knockout mutant (Δ ald ) of M. tuberculosis (JHU2780-209) was obtained from the Johns Hopkins TARGET mutant collection .

Techniques: In Vitro, Concentration Assay

Journal: Nature genetics

Article Title: Genomic and functional analyses of Mycobacterium tuberculosis strains implicate ald in D-cycloserine resistance

doi: 10.1038/ng.3548

Figure Lengend Snippet: A single gene knockout of ald confers a growth advantage relative to wild type M. tuberculosis when cultured in the presence of D-cycloserine. Four laboratory strains—wild type M. tuberculosis (WT), ald knockout (Δ ald ), ald complemented back into Δ ald ( ald -comp) and BCG—were cultured in triplicate in 0, 7.5 and 15 μg/mL of D-cycloserine. The resulting time to positivity (ttp) in MGIT was recorded as days since inoculation, and the resulting ttp was normalized to the no drug control for each strain to calculate the growth inhibition index for each drug concentration. The mean growth inhibition index is plotted with error bars to represent the SEM. P-values were calculated using a two-way ANOVA.

Article Snippet: An ald knockout mutant (Δ ald ) of M. tuberculosis (JHU2780-209) was obtained from the Johns Hopkins TARGET mutant collection .

Techniques: Gene Knockout, Cell Culture, Knock-Out, Control, Inhibition, Concentration Assay

Journal: Nature genetics

Article Title: Genomic and functional analyses of Mycobacterium tuberculosis strains implicate ald in D-cycloserine resistance

doi: 10.1038/ng.3548

Figure Lengend Snippet: Clinical strains with mutations in L-alanine dehydrogenase ( ald ) and alanine racemace ( alr ) exhibit increased resistance to D-cycloserine. A set of 44 clinical strains of M. tuberculosis with differing genotypes with respect to ald and alr were selected, representing wild type alleles (WT), loss-of-function mutations (LOF), nonsynonymous mutations (NSY) and promoter mutations (PRO). The minimum inhibitory concentration (MIC) to D-cycloserine was determined for each strain on Löwenstein–Jensen media. For wild type strains and strains with ald loss-of-function mutations, the median is shown as an open circle and quartiles are shown as connected bars. When compared to WT isolates, strains with ald loss-of-function had significantly increased resistance to D-cycloserine (p < 0.0002, Mann-Whitney U test).

Article Snippet: An ald knockout mutant (Δ ald ) of M. tuberculosis (JHU2780-209) was obtained from the Johns Hopkins TARGET mutant collection .

Techniques: Concentration Assay, MANN-WHITNEY

Journal: PLoS Genetics

Article Title: The activation of OsEIL1 on YUC8 transcription and auxin biosynthesis is required for ethylene-inhibited root elongation in rice early seedling development

doi: 10.1371/journal.pgen.1006955

Figure Lengend Snippet: (A) Plant morphology of Kitaake (KT) and rein7-1 at the heading stage. Bar = 10 cm. (B) Typical image of rolled leaf. (C) The 1000-grain weight. Each value is the average of 30–50 plants. (D) Phenotypes of KT and rein7-1 under salt stress. Control indicates that rice seedlings were grown under normal conditions, and NaCl indicates that seedlings were treated with 150 mM NaCl aqueous solution. Bar = 10 cm. (E) Survival rate after salt treatment in (D). Approximately 50–60 seedlings were used in each experiment. Bars indicate ± SD of three independent assays. ** indicates a significant difference compared to KT at P < 0.01. (F) Map-based cloning of the YUC8 / REIN7 gene. The locus was mapped to chromosome 3 within a 63 kb region between M0721 and M0728. ‘n’ indicates the number of samples used for map-based cloning, ‘M’ represents marker. (G) Mutation sites of two allelic mutants are indicated in the schematic diagram of the YUC8/REIN7 protein. (H) Functional complementation of the rein7 mutant. The KT, rein7-1 and complementary lines were treated with 10 ppm ethylene for 3 d under dark conditions. Bar = 10 mm.

Article Snippet: The YUC8 (Os03g0162000) T-DNA knockout mutant rein7-2 (PFG_1C-07050.R) is on a Hwayoung (HY) background, identified by PCR using the T-DNA right border primer RB (5'-CCACAGTTTTCGCGATCCAGACTG-3') and gene-specific primers flanking the insertion site (RP, 5'-ATTCTGGCATGGAAGTGAGC-3'), was obtained from the POSTECH Biotech Center [ ].

Techniques: Control, Cloning, Marker, Mutagenesis, Functional Assay

Journal: PLoS Genetics

Article Title: The activation of OsEIL1 on YUC8 transcription and auxin biosynthesis is required for ethylene-inhibited root elongation in rice early seedling development

doi: 10.1371/journal.pgen.1006955

Figure Lengend Snippet: (A) and (C) Ethylene-response phenotypes of rein7 in dark or in normal growth conditions. KT and Hwayoung (HY) are the wild types. Seedlings were grown in the dark or in normal growth conditions for 3 d in the absence (air) or presence of 10 ppm of ethylene. Bar = 10 mm. (B) and (D) Root length in (A) and (C), correspondingly. Each column is the average of 20–30 seedlings, and bars indicate ± SD. The experiment was repeated at least three times with similar results. ** indicates significant difference compared to air at P < 0.01.

Article Snippet: The YUC8 (Os03g0162000) T-DNA knockout mutant rein7-2 (PFG_1C-07050.R) is on a Hwayoung (HY) background, identified by PCR using the T-DNA right border primer RB (5'-CCACAGTTTTCGCGATCCAGACTG-3') and gene-specific primers flanking the insertion site (RP, 5'-ATTCTGGCATGGAAGTGAGC-3'), was obtained from the POSTECH Biotech Center [ ].

Techniques:

Journal: PLoS Genetics

Article Title: The activation of OsEIL1 on YUC8 transcription and auxin biosynthesis is required for ethylene-inhibited root elongation in rice early seedling development

doi: 10.1371/journal.pgen.1006955

Figure Lengend Snippet: (A) Ethylene response phenotypes of KT and inducible transgenic line (PER8-REIN7) seedlings grown in the dark for 3 d in the presence or absence of 2.5 μM estradiol. Bar = 10 mm. (B) Root length and (C) relative root length (ethylene-treated versus untreated in each genotype, respectively) in (A). Each column is the average of 20–30 seedlings, and bars indicate ± SD. The experiment was repeated at least three times with similar results. * and ** indicate significant difference compared to KT at P < 0.05 and P < 0.01.

Article Snippet: The YUC8 (Os03g0162000) T-DNA knockout mutant rein7-2 (PFG_1C-07050.R) is on a Hwayoung (HY) background, identified by PCR using the T-DNA right border primer RB (5'-CCACAGTTTTCGCGATCCAGACTG-3') and gene-specific primers flanking the insertion site (RP, 5'-ATTCTGGCATGGAAGTGAGC-3'), was obtained from the POSTECH Biotech Center [ ].

Techniques: Transgenic Assay

Journal: PLoS Genetics

Article Title: The activation of OsEIL1 on YUC8 transcription and auxin biosynthesis is required for ethylene-inhibited root elongation in rice early seedling development

doi: 10.1371/journal.pgen.1006955

Figure Lengend Snippet: (A) YUC8/REIN7 expression in different rice tissues detected by qPCR. Bars indicate ± SD from five independent experiments. (B) Tissue-specific expression of YUC8/REIN7 revealed by transgenic line (YUC8 promoter-GUS) analysis. (a) 3-d-old etiolated seedlings. (b) Root tip. Bar = 100 μm. (c) Root. (d) Young stem nodes. (e) Leaf. (f) Stem. (g) Developing grains. Bar = 10 mm.

Article Snippet: The YUC8 (Os03g0162000) T-DNA knockout mutant rein7-2 (PFG_1C-07050.R) is on a Hwayoung (HY) background, identified by PCR using the T-DNA right border primer RB (5'-CCACAGTTTTCGCGATCCAGACTG-3') and gene-specific primers flanking the insertion site (RP, 5'-ATTCTGGCATGGAAGTGAGC-3'), was obtained from the POSTECH Biotech Center [ ].

Techniques: Expressing, Transgenic Assay

Journal: PLoS Genetics

Article Title: The activation of OsEIL1 on YUC8 transcription and auxin biosynthesis is required for ethylene-inhibited root elongation in rice early seedling development

doi: 10.1371/journal.pgen.1006955

Figure Lengend Snippet: (A) The simplified liner pathway of IAA biosynthesis. The red arrows indicate the function of YUC. Enzymatic assays with GST-AtYUC2, GST- REIN7, GST-REIN7m and the product IAA (B), the substrate TAM (C) were analyzed by LC-ESI-MS/MS. The bars represent ± SD from three independent experiments. (D) The content of remained NADPH in (C). The bars represent ± SD from three independent experiments. (E) Expression of auxin-inducible genes in 7-d-old normal grown seedlings. The bars represent ± SD from five independent experiments. (F) IAA content of 7-d-old normal grown seedlings. The bars represent ± SD from three independent experiments. ** indicates a significant difference compared to KT or HY at P < 0.01.

Article Snippet: The YUC8 (Os03g0162000) T-DNA knockout mutant rein7-2 (PFG_1C-07050.R) is on a Hwayoung (HY) background, identified by PCR using the T-DNA right border primer RB (5'-CCACAGTTTTCGCGATCCAGACTG-3') and gene-specific primers flanking the insertion site (RP, 5'-ATTCTGGCATGGAAGTGAGC-3'), was obtained from the POSTECH Biotech Center [ ].

Techniques: Tandem Mass Spectroscopy, Expressing

Journal: PLoS Genetics

Article Title: The activation of OsEIL1 on YUC8 transcription and auxin biosynthesis is required for ethylene-inhibited root elongation in rice early seedling development

doi: 10.1371/journal.pgen.1006955

Figure Lengend Snippet: (A) DR5-GUS expression in root. Seedlings of 3-d-old etiolated transgenic lines containing DR5-GUS in the wild type or the rein7-1 background were treated with or without 10 ppm ethylene for 8 h before GUS activity was assayed. Bar = 10mm. (B) Root tip and elongation zone in (A). ‘RT’ represents root tip, ‘EZ’ represents elongation zone. Bar = 100 μm. (C) qPCR analysis of auxin-response gene expression in response to ethylene. Dark-grown 3-d-old wild-type seedlings were treated with 10 ppm ethylene for 3 h. The RNAs from roots were isolated for qPCR. (D) IAA levels in 3-d-old wild-type and rein7 etiolated seedlings in the absence or presence of 10 ppm ethylene. (E) Rescue of the reduced ethylene sensitivity of rein7-1 root by IAA. The wild-type and rein7-1 seedlings were grown in the dark for 3 d in the absence or presence of 10 ppm ethylene, with or without supplementation of 10 nM IAA. Bar = 10 mm. (F) Quantification of root inhibition in (D). Each column is the average of 20–30 seedlings. The data are shown as the mean ± SD of three biological replicates. * and ** indicate significant differences between the compared two samples at P < 0.05 and P < 0.01, respectively.

Article Snippet: The YUC8 (Os03g0162000) T-DNA knockout mutant rein7-2 (PFG_1C-07050.R) is on a Hwayoung (HY) background, identified by PCR using the T-DNA right border primer RB (5'-CCACAGTTTTCGCGATCCAGACTG-3') and gene-specific primers flanking the insertion site (RP, 5'-ATTCTGGCATGGAAGTGAGC-3'), was obtained from the POSTECH Biotech Center [ ].

Techniques: Expressing, Transgenic Assay, Activity Assay, Gene Expression, Isolation, Inhibition

Journal: PLoS Genetics

Article Title: The activation of OsEIL1 on YUC8 transcription and auxin biosynthesis is required for ethylene-inhibited root elongation in rice early seedling development

doi: 10.1371/journal.pgen.1006955

Figure Lengend Snippet: (A) Expression of YUC8 / REIN7 in response to ethylene. The wild-type seedlings were grown in the dark for 3 d and then treated with 10 ppm ethylene. The RNAs from roots and shoots were isolated and used for qPCR. (B) Ethylene-induced GUS activity in roots of transgenic plants harboring REIN7p-GUS . Etiolated seedlings of 3-d-old plants were treated with or without 10 ppm ethylene for 8 h before GUS activity was assayed. Bar = 10mm. (C) Root tip and elongation zone in (B). ‘RT’ represents root tip, ‘EZ’ represents elongation zone. Bar = 100 μm. (D) qPCR analysis of YUC8 / REIN7 expression in primary roots and shoots of Nip, oseil1 and EIL1-OX seedlings grown in the dark for 3 d. (E) Expression of YUC8 / REIN7 in primary roots of Nip, oseil1 and EIL1-OX seedlings grown in the dark for 3 d and then treated with 10 ppm ethylene. The data are shown as the mean ± SD of five biological replicates. * and ** indicate significant differences compared to 0 h at P < 0.05 and P < 0.01, respectively.

Article Snippet: The YUC8 (Os03g0162000) T-DNA knockout mutant rein7-2 (PFG_1C-07050.R) is on a Hwayoung (HY) background, identified by PCR using the T-DNA right border primer RB (5'-CCACAGTTTTCGCGATCCAGACTG-3') and gene-specific primers flanking the insertion site (RP, 5'-ATTCTGGCATGGAAGTGAGC-3'), was obtained from the POSTECH Biotech Center [ ].

Techniques: Expressing, Isolation, Activity Assay, Transgenic Assay

Journal: PLoS Genetics

Article Title: The activation of OsEIL1 on YUC8 transcription and auxin biosynthesis is required for ethylene-inhibited root elongation in rice early seedling development

doi: 10.1371/journal.pgen.1006955

Figure Lengend Snippet: (A) Schematic diagrams of putative EIN3 binding site (EBS) in the promoter of YCU8/REIN7 . Black boxes indicate the positions of the EBS. P1-P4 are fragments of the YUC8/REIN7 promoter. (B) Anti-myc ChIP assays with DNA from 3-d-old etiolated seedling roots of Nip and overexpressing OsEIL1 with myc-tag (EIL1-myc) transgenic plants. (C) EMSA assay for binding to EBS sequence in the promoter of YUC8/REIN7 by OsEIL1 protein in vitro . Glutathione S -transferase (GST)-tagged OsEIL1 N-terminal fusion protein was incubated with biotin-labeled DNA fragments (Probe). Competition for the biotin-labeled promoter region was done by adding an excess of unlabeled probe (Competitor). Three biological replicates were performed with similar results. (D) The activation of OsEIL1 on the promoter activity of YUC8/REIN7 by transient expression assay in tobacco leaves. ‘EV’ represents empty vector. Three biological replicates were performed with similar results. (E) Quantitative analysis of luminescence intensity for each comparison in (D).

Article Snippet: The YUC8 (Os03g0162000) T-DNA knockout mutant rein7-2 (PFG_1C-07050.R) is on a Hwayoung (HY) background, identified by PCR using the T-DNA right border primer RB (5'-CCACAGTTTTCGCGATCCAGACTG-3') and gene-specific primers flanking the insertion site (RP, 5'-ATTCTGGCATGGAAGTGAGC-3'), was obtained from the POSTECH Biotech Center [ ].

Techniques: Binding Assay, Transgenic Assay, Sequencing, In Vitro, Incubation, Labeling, Activation Assay, Activity Assay, Expressing, Plasmid Preparation, Quantitative Luminescence, Comparison

Journal: PLoS Genetics

Article Title: The activation of OsEIL1 on YUC8 transcription and auxin biosynthesis is required for ethylene-inhibited root elongation in rice early seedling development

doi: 10.1371/journal.pgen.1006955

Figure Lengend Snippet: (A) Phenotypes of Nip, oseil1 , oseil1 REIN7-OX and REIN7-OX dark-grown seedlings in the presence or absence of 10 ppm ethylene for 3 d. Bars = 10 mm. (B) Root length and relative root length (ethylene-treated versus untreated in each genotype, respectively) in (A). (C) Phenotypes of KT, rein7-1 , rein7-1 EIL1-OX, EIL1-OX and Nip dark-grown seedlings in the presence or absence of 10 ppm ethylene for 3 d. Bars = 10 mm. (D) Root length and relative root length (ethylene-treated versus untreated in each genotype, respectively) in (C). In (B) and (D), each column is the average of 20–30 seedlings, and bars indicate ± SD. * and ** indicates a significant difference compared to air at P < 0.05 and P < 0.01. (E) A proposed model of ethylene-inhibited primary root elongation in rice. Ethylene signaling acts upstream of the auxin biosynthesis to regulate primary root elongation. YUC8/REIN7 is a key regulator required for ethylene-inhibited primary root elongation.

Article Snippet: The YUC8 (Os03g0162000) T-DNA knockout mutant rein7-2 (PFG_1C-07050.R) is on a Hwayoung (HY) background, identified by PCR using the T-DNA right border primer RB (5'-CCACAGTTTTCGCGATCCAGACTG-3') and gene-specific primers flanking the insertion site (RP, 5'-ATTCTGGCATGGAAGTGAGC-3'), was obtained from the POSTECH Biotech Center [ ].

Techniques:

Journal: The Plant Cell

Article Title: The WRKY6 Transcription Factor Modulates PHOSPHATE1 Expression in Response to Low Pi Stress in Arabidopsis [W] [OA]

doi: 10.1105/tpc.108.064980

Figure Lengend Snippet: Repression of PHO1 Expression by WRKY6 Was Released in Response to Low Pi Stress.

Article Snippet: We thank Imre E. Somssich (Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Germany) for kindly providing At WRKY6 overexpression lines 35S: WRKY6-3 , 35S: WRKY6-5 , and 35S: WRKY6-9 and the At WRKY6 knockout mutant ( wrky6-1 ).

Techniques: Expressing

Journal: The Plant Cell

Article Title: The WRKY6 Transcription Factor Modulates PHOSPHATE1 Expression in Response to Low Pi Stress in Arabidopsis [W] [OA]

doi: 10.1105/tpc.108.064980

Figure Lengend Snippet: ChIP Assays for At WRKY6 Binding to the W-Box of the PHO1 Promoter in Vivo.

Article Snippet: We thank Imre E. Somssich (Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Germany) for kindly providing At WRKY6 overexpression lines 35S: WRKY6-3 , 35S: WRKY6-5 , and 35S: WRKY6-9 and the At WRKY6 knockout mutant ( wrky6-1 ).

Techniques: Binding Assay, In Vivo

Journal: The Plant Cell

Article Title: The WRKY6 Transcription Factor Modulates PHOSPHATE1 Expression in Response to Low Pi Stress in Arabidopsis [W] [OA]

doi: 10.1105/tpc.108.064980

Figure Lengend Snippet: Suppression of PHO1 Expression by WRKY6.

Article Snippet: We thank Imre E. Somssich (Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Germany) for kindly providing At WRKY6 overexpression lines 35S: WRKY6-3 , 35S: WRKY6-5 , and 35S: WRKY6-9 and the At WRKY6 knockout mutant ( wrky6-1 ).

Techniques: Expressing

Journal: The Plant Cell

Article Title: The WRKY6 Transcription Factor Modulates PHOSPHATE1 Expression in Response to Low Pi Stress in Arabidopsis [W] [OA]

doi: 10.1105/tpc.108.064980

Figure Lengend Snippet: ChIP-qPCR Assays to Detect the Association of WRKY6 and the PHO1 Promoter in the Tested Plants as Indicated under Pi-Sufficient (MS) and Pi-Deficient (LP) Conditions.

Article Snippet: We thank Imre E. Somssich (Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Germany) for kindly providing At WRKY6 overexpression lines 35S: WRKY6-3 , 35S: WRKY6-5 , and 35S: WRKY6-9 and the At WRKY6 knockout mutant ( wrky6-1 ).

Techniques: ChIP-qPCR

Journal: The Plant Cell

Article Title: The WRKY6 Transcription Factor Modulates PHOSPHATE1 Expression in Response to Low Pi Stress in Arabidopsis [W] [OA]

doi: 10.1105/tpc.108.064980

Figure Lengend Snippet: WRKY6 Protein Blot Analysis.

Article Snippet: We thank Imre E. Somssich (Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Germany) for kindly providing At WRKY6 overexpression lines 35S: WRKY6-3 , 35S: WRKY6-5 , and 35S: WRKY6-9 and the At WRKY6 knockout mutant ( wrky6-1 ).

Techniques: